Purification:Article Title: Development of a chimeric cytokine receptor that captures IL-6 and enhances the antitumor response of CAR-T cells.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Human IL-6 Enhanced Sensitivity Flex Set BD Biosciences 561512 CellTrace CFSE Cell Proliferation Kit Thermo Fisher Scientific C34554 LIVE/DEAD Fixable Near-IR Dead Cell Stain Kit Thermo Fisher Scientific L34976 Cyto-Fast Fix/Perm Buffer Set BioLegend 426803 PE-Cy7 Annexin V BioLegend 640951 Maxpar X8 Antibody labeling kit, 151Eu- 4Rxn Standard BioTools 201151A Maxpar X8 Antibody Labeling Kit, 155Gd—4 Rxn Standard BioTools 201155A Maxpar X8 Antibody Labeling Kit, 158Gd—4 Rxn Standard BioTools 201158A Deposited data RNA-seq of CAR-T cells (Figure 2) This paper GSE216734 Mass cytometry analysis of CAR-T cells This paper FlowRepository (http://flowrepository.org): FR-FCM-Z77Y RNA-seq of CAR-T cells (Figure S3) This paper GSE255145 Experimental models: Cell lines K562 Japanese Collection of Research Bioresources (JCRB) JCRB0019 Raji Japanese Collection of Research Bioresources (JCRB) JCRB9012 NALM-6 Cell Resource Center for Biomedical Research TKG 0413 PG13 American Type Culture Collection (ATCC) CRL-3597 AsPC-1 American Type Culture Collection (ATCC) CRL-1682 Jurkat, clone E6-1 American Type Culture Collection (ATCC) TIB-152 Plat-A Toshio Kitamura N/A Plat-E Toshio Kitamura N/A Lenti-X 293T Takara Bio Cat#632180 K562-OKT3/CD80 Yoshikawa et al.59 N/A K562-CD19 Yoshikawa et al.59 N/A K562-mesothelin Yoshikawa et al.59 N/A NALM6-GL Yoshikawa et al.59 N/A NALM6-GD2 Yoshikawa et al.59 N/A Experimental models: Organisms/strains NOD-SCID IL2rgnull (NSG) Jackson Laboratory Strain #:005557 NOG-Iab KO (NOG-DMHC) In-Vivo Science N/A NOG-hGM-CSF/hIL-3 Tg (NOG-EXL) In-Vivo Science N/A Recombinant DNA pMX Toshio Kitamura N/A Anti-CD19 scFv (clone FMC63) Nicholson et al.60 N/A Anti-mesothelin scFv (clone ss1) Li et al.61 N/A Anti-GD2 scFv (clone 14g2a) with E101K mutation Rossig et al.62 Horwacik et al.63 N/A (Continued on next page) Cell Reports Medicine 5, 101526, May 21, 2024 e3 OPEN ACCESS
Virus:Article Title: Development of a chimeric cytokine receptor that captures IL-6 and enhances the antitumor response of CAR-T cells.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Human IL-6 Enhanced Sensitivity Flex Set BD Biosciences 561512 CellTrace CFSE Cell Proliferation Kit Thermo Fisher Scientific C34554 LIVE/DEAD Fixable Near-IR Dead Cell Stain Kit Thermo Fisher Scientific L34976 Cyto-Fast Fix/Perm Buffer Set BioLegend 426803 PE-Cy7 Annexin V BioLegend 640951 Maxpar X8 Antibody labeling kit, 151Eu- 4Rxn Standard BioTools 201151A Maxpar X8 Antibody Labeling Kit, 155Gd—4 Rxn Standard BioTools 201155A Maxpar X8 Antibody Labeling Kit, 158Gd—4 Rxn Standard BioTools 201158A Deposited data RNA-seq of CAR-T cells (Figure 2) This paper GSE216734 Mass cytometry analysis of CAR-T cells This paper FlowRepository (http://flowrepository.org): FR-FCM-Z77Y RNA-seq of CAR-T cells (Figure S3) This paper GSE255145 Experimental models: Cell lines K562 Japanese Collection of Research Bioresources (JCRB) JCRB0019 Raji Japanese Collection of Research Bioresources (JCRB) JCRB9012 NALM-6 Cell Resource Center for Biomedical Research TKG 0413 PG13 American Type Culture Collection (ATCC) CRL-3597 AsPC-1 American Type Culture Collection (ATCC) CRL-1682 Jurkat, clone E6-1 American Type Culture Collection (ATCC) TIB-152 Plat-A Toshio Kitamura N/A Plat-E Toshio Kitamura N/A Lenti-X 293T Takara Bio Cat#632180 K562-OKT3/CD80 Yoshikawa et al.59 N/A K562-CD19 Yoshikawa et al.59 N/A K562-mesothelin Yoshikawa et al.59 N/A NALM6-GL Yoshikawa et al.59 N/A NALM6-GD2 Yoshikawa et al.59 N/A Experimental models: Organisms/strains NOD-SCID IL2rgnull (NSG) Jackson Laboratory Strain #:005557 NOG-Iab KO (NOG-DMHC) In-Vivo Science N/A NOG-hGM-CSF/hIL-3 Tg (NOG-EXL) In-Vivo Science N/A Recombinant DNA pMX Toshio Kitamura N/A Anti-CD19 scFv (clone FMC63) Nicholson et al.60 N/A Anti-mesothelin scFv (clone ss1) Li et al.61 N/A Anti-GD2 scFv (clone 14g2a) with E101K mutation Rossig et al.62 Horwacik et al.63 N/A (Continued on next page) Cell Reports Medicine 5, 101526, May 21, 2024 e3 OPEN ACCESS
Recombinant:Article Title: Development of a chimeric cytokine receptor that captures IL-6 and enhances the antitumor response of CAR-T cells.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Human IL-6 Enhanced Sensitivity Flex Set BD Biosciences 561512 CellTrace CFSE Cell Proliferation Kit Thermo Fisher Scientific C34554 LIVE/DEAD Fixable Near-IR Dead Cell Stain Kit Thermo Fisher Scientific L34976 Cyto-Fast Fix/Perm Buffer Set BioLegend 426803 PE-Cy7 Annexin V BioLegend 640951 Maxpar X8 Antibody labeling kit, 151Eu- 4Rxn Standard BioTools 201151A Maxpar X8 Antibody Labeling Kit, 155Gd—4 Rxn Standard BioTools 201155A Maxpar X8 Antibody Labeling Kit, 158Gd—4 Rxn Standard BioTools 201158A Deposited data RNA-seq of CAR-T cells (Figure 2) This paper GSE216734 Mass cytometry analysis of CAR-T cells This paper FlowRepository (http://flowrepository.org): FR-FCM-Z77Y RNA-seq of CAR-T cells (Figure S3) This paper GSE255145 Experimental models: Cell lines K562 Japanese Collection of Research Bioresources (JCRB) JCRB0019 Raji Japanese Collection of Research Bioresources (JCRB) JCRB9012 NALM-6 Cell Resource Center for Biomedical Research TKG 0413 PG13 American Type Culture Collection (ATCC) CRL-3597 AsPC-1 American Type Culture Collection (ATCC) CRL-1682 Jurkat, clone E6-1 American Type Culture Collection (ATCC) TIB-152 Plat-A Toshio Kitamura N/A Plat-E Toshio Kitamura N/A Lenti-X 293T Takara Bio Cat#632180 K562-OKT3/CD80 Yoshikawa et al.59 N/A K562-CD19 Yoshikawa et al.59 N/A K562-mesothelin Yoshikawa et al.59 N/A NALM6-GL Yoshikawa et al.59 N/A NALM6-GD2 Yoshikawa et al.59 N/A Experimental models: Organisms/strains NOD-SCID IL2rgnull (NSG) Jackson Laboratory Strain #:005557 NOG-Iab KO (NOG-DMHC) In-Vivo Science N/A NOG-hGM-CSF/hIL-3 Tg (NOG-EXL) In-Vivo Science N/A Recombinant DNA pMX Toshio Kitamura N/A Anti-CD19 scFv (clone FMC63) Nicholson et al.60 N/A Anti-mesothelin scFv (clone ss1) Li et al.61 N/A Anti-GD2 scFv (clone 14g2a) with E101K mutation Rossig et al.62 Horwacik et al.63 N/A (Continued on next page) Cell Reports Medicine 5, 101526, May 21, 2024 e3 OPEN ACCESS
Enzyme-linked Immunosorbent Assay:Article Title: Development of a chimeric cytokine receptor that captures IL-6 and enhances the antitumor response of CAR-T cells.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Human IL-6 Enhanced Sensitivity Flex Set BD Biosciences 561512 CellTrace CFSE Cell Proliferation Kit Thermo Fisher Scientific C34554 LIVE/DEAD Fixable Near-IR Dead Cell Stain Kit Thermo Fisher Scientific L34976 Cyto-Fast Fix/Perm Buffer Set BioLegend 426803 PE-Cy7 Annexin V BioLegend 640951 Maxpar X8 Antibody labeling kit, 151Eu- 4Rxn Standard BioTools 201151A Maxpar X8 Antibody Labeling Kit, 155Gd—4 Rxn Standard BioTools 201155A Maxpar X8 Antibody Labeling Kit, 158Gd—4 Rxn Standard BioTools 201158A Deposited data RNA-seq of CAR-T cells (Figure 2) This paper GSE216734 Mass cytometry analysis of CAR-T cells This paper FlowRepository (http://flowrepository.org): FR-FCM-Z77Y RNA-seq of CAR-T cells (Figure S3) This paper GSE255145 Experimental models: Cell lines K562 Japanese Collection of Research Bioresources (JCRB) JCRB0019 Raji Japanese Collection of Research Bioresources (JCRB) JCRB9012 NALM-6 Cell Resource Center for Biomedical Research TKG 0413 PG13 American Type Culture Collection (ATCC) CRL-3597 AsPC-1 American Type Culture Collection (ATCC) CRL-1682 Jurkat, clone E6-1 American Type Culture Collection (ATCC) TIB-152 Plat-A Toshio Kitamura N/A Plat-E Toshio Kitamura N/A Lenti-X 293T Takara Bio Cat#632180 K562-OKT3/CD80 Yoshikawa et al.59 N/A K562-CD19 Yoshikawa et al.59 N/A K562-mesothelin Yoshikawa et al.59 N/A NALM6-GL Yoshikawa et al.59 N/A NALM6-GD2 Yoshikawa et al.59 N/A Experimental models: Organisms/strains NOD-SCID IL2rgnull (NSG) Jackson Laboratory Strain #:005557 NOG-Iab KO (NOG-DMHC) In-Vivo Science N/A NOG-hGM-CSF/hIL-3 Tg (NOG-EXL) In-Vivo Science N/A Recombinant DNA pMX Toshio Kitamura N/A Anti-CD19 scFv (clone FMC63) Nicholson et al.60 N/A Anti-mesothelin scFv (clone ss1) Li et al.61 N/A Anti-GD2 scFv (clone 14g2a) with E101K mutation Rossig et al.62 Horwacik et al.63 N/A (Continued on next page) Cell Reports Medicine 5, 101526, May 21, 2024 e3 OPEN ACCESS
|
fluidigm is a verified supplier 
fluidigm manufactures this product 